4.2. EGCG-S and Antibiotic Formulations
This protocol is extracted from research article:
Inhibition of Biofilm Formation by the Synergistic Action of EGCG-S and Antibiotics
Antibiotics (Basel), Jan 21, 2021; DOI: 10.3390/antibiotics10020102

EGCG-S (US Patent 8076484) purchased from Camellix LLC (Evans, GA, USA), was dissolved in absolute ethanol to make a stock concentration of 10 mg/mL prior to formulations. The stock was diluted to the required concentrations for each experiment. Phosphate buffer saline (PBS) was used as a negative control, while 10% bleach was used as a positive control. Antibiotics erythromycin (E0774) and tetracycline (T3258), were purchased from Sigma Aldrich (St. Louis, MO, USA). The stock concentration (1000 μg/mL) was prepared by dissolving antibiotics in absolute ethanol. Final concentrations of ethanol in the working solutions were all less than 5%, which did not inhibit the growth of the bacteria. The solutions were filter sterilized and stored at −20 °C. The required concentrations were diluted from the stock aseptically prior to the experiment. The formulations consisted of varying concentrations of EGCG-S and antibiotics depending on the selected bacterium.

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