4.1. Bacterial Cultures
This protocol is extracted from research article:
Inhibition of Biofilm Formation by the Synergistic Action of EGCG-S and Antibiotics
Antibiotics (Basel), Jan 21, 2021; DOI: 10.3390/antibiotics10020102

Five potential pathogenic biofilm producers, Escherichia coli (ATCC® CRM-8739), Pseudomonas aeruginosa (ATCC® CRM-9027), Staphylococcus aureus (ATCC® CRM-6538), Staphylococcus epidermidis (ATCC® 14990), and Mycobacterium smegmatis (ATCC® 19420) were grown aseptically on nutrient agar or broth. The stock cultures were stored at 4 °C. Fresh overnight cultures were maintained at 37 °C with constant shaking at 250 rpm. Gram staining was performed before each experiment to confirm the culture purity.

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