To assess heat tolerance of microencapsulated L. reuteri DSM 17938 and L. plantarum 48M cells, bacteria survival was tested against four time-temperature combinations: 60 °C for 3 min, 70 °C for 2 min, 80 °C for 2 min, 80 °C for 5 min. Samples were prepared as follows: free or microencapsulated cells were 10-fold diluted in MRS Broth and sealed in glass capillary tubes (Ø 2 mm). The tubes were then placed in a thermostatic water bath and treated at the conditions cited above. At the end of the period, glass tubes were removed and rapidly cooled under running tap water.

Aliquots were sampled before and after thermal treatments and counted by serially diluting 1 mL aliquots of the test material, pour plating on MRS Agar and incubating at optimal growth temperature for the tested strain for 48 h. Results were expressed in terms of surviving percentage as Nt/N0 × 100, where N0 and Nt are cell loads expressed in CFU/mL before and after heat exposure, respectively. Three independent experiments were carried out.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.