Values are reported as the mean ± standard deviation or a 95% CI range. Paired t-tests were used to compare the body weight and body composition between day 1 and 4, and whether the energy balance on day 1, 2, and 3, or the NBAL on day 3 was positive or negative. Linear regression analyses were applied to determine the relationship between the protein intake and the NBAL. Data were analyzed using the GraphPad Prism 6 software (GraphPad Software Inc., San Diego, CA, USA); p < 0.05 was considered significant.

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