The urine samples were stored at 4 °C until analysis after acidification with anhydrous citric acid. The creatinine and urea concentrations in the urine were measured by SRL Medisearch, Inc. (Shinjuku, Tokyo, Japan). Nitrogen excretion was calculated by summing the urinary excretion of urea-bound nitrogen and creatinine-bound nitrogen, which represents >75% of the total nitrogen excretion in athletes [11]. NBAL was estimated as follows: NBAL = E − I, where E represents nitrogen excretion and I represents dietary nitrogen intake, calculated as the intake of protein and amino acids on day 3 divided by a nitrogen-to-protein conversion factor (6.25).

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.