2.6. Imaging and Analysis of Paper Sensor
This protocol is extracted from research article:
A Paper-Based Colorimetric Aptasensor for the Detection of Gentamicin
Biosensors (Basel), Jan 21, 2021; DOI: 10.3390/bios11020029

To estimate the LOD on the modified paper device, gentamicin-spiked buffer samples and milk samples were used. The paper experiment was repeated at least 3 times following the protocol illustrated in Section 2.4. The camera-based LOD for the paper sensor used the images captured on the 12 MP rear camera of iPhone 11, using a white background. RGB color analysis was performed on the obtained images (without modification) using Image J software 1.8 [27]. The images were loaded and split into individual channels of red (R), green (G) and blue (B). The reaction zone was analyzed for its red and blue intensities for both spiked TE and milk samples. The color intensity of both sample sets were plotted as a function of B/R vs concentration(nM), where B and R were blue and red intensities per pixel area of the reaction zone. The camera- based LOD was estimated using formula 3(standard deviation)/slope [28].

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