Each subject received an explanation regarding the purpose of the study, experimental protocol, and all potential risks before taking part in this study. The experimental protocol was conducted in accordance with the Declaration of Helsinki, and was approved by the research ethics board of the National Institute of Fitness and Sports (8–61), as well as the institutional review board of Ajinomoto Co., Inc. (Tokyo, Japan) (2017-015). Subjects aged >20 years provided written informed consent, while in cases of subjects aged 18–19 years, both the subjects and their parents provided written informed consent. This trial was registered at www.umin.ac.jp/ctr/index.htm (UMIN 000029374).

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