2.2. Gold Nanoparticle Synthesis and Characterization
This protocol is extracted from research article:
A Paper-Based Colorimetric Aptasensor for the Detection of Gentamicin
Biosensors (Basel), Jan 21, 2021; DOI: 10.3390/bios11020029

Gold nanoparticles were prepared by a single step citrate reduction method. All glassware and stir-bars were cleaned thoroughly and oven dried before use. In a typical synthesis, 1 mM of HAuCl4 was dissolved in MilliQ water and constantly stirred while it was brought to a rolling boil. Next, 38.8 mM trisodium citrate dihydrate was rapidly added to the boiling mixture. A significant color change from pale yellow to light blue to wine red was observed. The heat was turned off and allowed to stir for 2 h until the colloidal gold sol reached room temperature. The prepared AuNPs were stored for later use, in the dark at 4 °C [24]. Spectral analysis of the synthesized AuNPs were performed using Synergy H1 Multimodal Plate reader (Biotek® Instruments Inc., Winooski, VT, USA). The size and structure of the nanoparticles were analyzed using transmission electron microscope (FEI Tecnai G2 F20, San Francisco, CA, USA).

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