The effect of SEO and NEs on established biofilms was evaluated after 24 h bacterial growth in polystyrene 96-well plates at 37 °C. After this incubation time, the supernatant was removed by aspiration, and replaced with medium with SEO or NEs added at sub-inhibitory concentrations and 0.02% of Tween-80. After incubation with the substances for 24 h a 37 °C, unattached bacterial cells were removed, wells were rinsed twice with PBS, and stained with crystal violet (2% w/v), as previously described. Absorbance was measured at 570 nm with a microplate reader (Bio-rad Benchmark, Hercules, CA, USA).

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.