Western blot analysis was performed as described previously [13, 14], with primary antibodies including the following: rabbit anti-PDIA6 (1 : 1,500; Abcam, Cambridge, MA, USA), anti-beta-centractin (1 : 500; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA), and anti-β-actin antibody (1 : 2,000; Cell Signaling Technology, Beverly, MA, USA). HRP-conjugated secondary antibody (Santa Cruz Biotechnology) was used as the secondary antibody. The protein signals were detected using SuperSignal (R) West Femto Maximum Sensitivity Substrate (Thermo Scientific Pierce). The grayscale value quantified using ImageJ software was used to calculate relative protein expression. The assays were independently repeated at least three times.

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