C6 control, C6-A1-3, C6-A2-3, and C6-A3-3 cells (1 × 106 cells/well) were seeded until confluence in 24-well plates. A straight scratch was gently made using a 200 μl pipette tip, and the cells were cultured in serum-free medium. Images were captured 24 h after scratch generation using an inverted phase-contrast microscope (Leica, Germany), and the area of the wound was quantified using ImageJ software. The assays were independently repeated at least three times.

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