C6 control, C6-A1-3, C6-A2-3, and C6-A3-3 cells were plated in triplicate into 96-well culture plates (2,000 cells/well) in growth medium and cultured at 37°C and 5% CO2. Next, 10 μl/well of reagent (cell counting kit-8 (CCK-8); CK04, Dojindo, Japan) was added at 24, 48, 72, and 96 h after plating. Optical densities at a wavelength of 450 nm (OD450) were measured after 3 h of CCK-8 incubation at 37°C and 5% CO2, using a microplate reader (Wellscan MK3, Thermo LabSystems, Finland). The assays were independently repeated at least three times.

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