The protein levels of IL-1β, IL-18, IL-6, MCP-1, and TNF-α in the perihematomal brain samples were detected by the IL-1β ELISA kit (Abcam, Cambridge, UK), IL-18 ELISA kit (MBL, Nagoya, Japan), IL-6 ELISA kit, MCP-1 ELISA kit, and TNF-α ELISA kit (all three from R&D Systems, Minneapolis, MN, USA) according to the manufacturer's instructions. A spectrophotometer was employed to measure the 450 nm absorbance value.

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