Evans blue extravasation assay was performed to assess the permeability of the blood-brain barrier (BBB) after ICH. In brief, a 2% Evans blue (4 ml/kg, Sigma, St. Louis, MO, USA) solution in saline was administered into the mice tail vein 3 h before sacrifice on day 3 after operation. After 3 h circulation, animals were deeply anesthetized and perfused with cold saline through the left ventricle until the colorless fluid outflowed from the right atrium. Afterward, the ipsilateral and contralateral hemispheres were rapidly collected. Then, the hemispheres were weighed and homogenized in 1 ml of 50% ice-cold trichloroacetic acid (Sigma, St. Louis, MO, USA). After centrifugation, the supernatant was collected and mixed with ethanol (1 : 3). A spectrophotometer at a wavelength of 610 nm was used to measure the absorbance. The data were expressed as Evans blue (μg)/tissue (g).

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.