Drosophila strains and culture conditions

UAS-Rab5DN, UAS-Rab7DN, UAS-Rab11DN, UAS-Rab5WT, UAS-Rab11CA, puc-lacZ, UAS-hepAct, UAS-RasV12, UAS-bsk, UAS-Atg1 #2, UAS-p35, Rab5-Gal4, Rab11-Gal4, UAS-GFP, ppl-Gal4, and da-Gal4 were obtained from the Bloomington Drosophila Stock Center (BDSC). UAS-Rab5 RNAi, UAS-Rab7 RNAi, UAS-Rab11 RNAi, UAS-bsk RNAi, UAS-hep RNAi, UAS-dorsal RNAi, UAS-Dif RNAi, UAS-Atg1 RNAi, UAS-Atg8 RNAi, and UAS-hop RNAi were obtained from the Vienna Drosophila Resource Center. Hml-Gal4, UAS-jra RNAi, UAS-kay RNAi, UAS-dFOXO RNAi, UAS-Ras85d RNAi, UAS-Syx17 RNAi, elav-Gal4, and Tub-Gal4; Tub-Gal80ts were obtained from the Tsinghua Fly Center. Hml-Gal4;UAS-2xEGFP, UAS-STAT92E, UAS-Adgf-A, and Antp-Gal4 were gifts from Utpal Banerjee (Mandal et al., 2007); col-Gal4 UAS-mCD8GFP was a gift from Lucas Waltzer (Benmimoun et al., 2015); the srp-Gal4, domeMESO-lacZ, and dome-Gal4;UAS-2xEGFP lines were gifts from Jiwon Shim; and pxn-Gal4;UAS-GFP and He-Gal4;UAS-GFP were gifts from Norbert Perrimon. UAS-puc, UAS-bskDN, UAS-TRAF1 RNAi, and UAS-TAK1 RNAi were gifts from José Carlos Pastor-Pareja (Willsey et al., 2016). Cg;Atg8a-mCherry and Cg;p62-HA were gifts from Chao Tong. The UAS-Atg1 #3 line was gifted by Guangchao Chen (Chen et al., 2008). The UAS-dFOXO line was a gift from Pierre Léopold. The NP3084-Gal4 was a gift from Bing Zhou (Xiao et al., 2019). The w1118line (from BDSC) was used as the wild-type (WT) line in this article. For crosses performed with the UAS/Gal4 system, flies were allowed to lay eggs at 25°C; after the eggs hatched into first instar larvae, the larvae were transferred to 29°C. The other strains were reared at 25°C. All strains and crosses were cultured on standard cornmeal-yeast medium.

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