We plated 50,000 cells per well of a 48-well plate. The cells were transduced the following morning using the indicated pseudotyped lentiviral amounts plus media supplemented with polybrene 8 μg/mL to a final volume of 150 μL per well. The media was changed 8 hr post-transduction. The cells were analyzed by flow cytometry 72 hr post-transduction.

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