The study involved 40 volunteers who were interviewed and had a medical examination to exclude any contraindications for cryostimulation. The inclusion criteria consisted of: BMI in the normal range (NW, normal weight group) and BMI over 35 (OB, obese group), age 20–35 years, male gender, no contraindications for systemic cryostimulation procedures and consent by the qualifying doctor to participate in the study. Exclusion criteria were: taking anti-inflammatory drugs and supplements containing vitamins and antioxidants. Glycemic levels allowing the diagnosis of diabetes were also one of exclusion criterium. Each procedure was preceded by blood pressure control (threshold value preventing the continuation of participation in the project: 160/100 mmHg). Respondents were asked to maintain their nutrition habits and recreational physical activity levels. A full protocol was completed by 24 young men (Figure 1): 14 with high and 10 with normal body weight. Study group characteristics are shown in Table1. The reasons for falling out of participants were: high blood pressure before entering cryochamber, respiratory tract infections, cold intolerance, resignation without giving any reason.

Patient flow diagram. A – age, BH – body height, NW – volunteers with normal BMI, OB – volunteers with BMI> 35.

Anthropometric characteristics of the subjects.

NW – volunteers with normal BMI, OB – volunteers with BMI> 35, A – age, BH – body height, BM – body mass, BMI – body mass index, PBF – percentage of body fat; #statistically significant differences between studied groups (p < 0.05)

The respondents, in accordance with the Declaration of Helsinki, were informed about the purpose and method of research, the possibility of resignation at each stage of the project without any consequences, provided insight into their results and gave their written consent to participate in the study. The consent of the Bioethics Committee at the Regional Medical Chamber in Krakow (number 122/KBL/OIL/2017) was obtained for conducting this research. This project obtained the trial registered number on ANZCTR (ACTRN12619000524190, date: 2.04.2019).

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