All statistical analyses were performed using the GraphPad Prism software (version 5.0; GraphPad Software, Inc.). Data are expressed as the means ± standard deviation. All data were tested for normality and equal variance and were proved to be consistent with variance with R studio software. The Student's t-test was used to compare the statistical differences between 2 groups. One-way ANOVA or two-way ANOVA followed by Tukey's post hoc test was used for comparisons among >2 groups. All experiments were repeated independently at least 3 times. A value of P≤0.05 was considered to indicate a statistically significant difference.

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