For IHC staining, paraffin-embedded ileal tissue (3-μm thickness) was heated, deparaffinized using xylene, and rehydrated in a graded series of ethanol. Antigen retrieval was performed and hydrogen peroxide was used to inhibit endogenous peroxidase activity. Sections were then incubated with primary antibody at 4°C overnight and HRP-conjugated rabbit IgG for 20 min. Color development time was controlled under the microscope. Samples were dehydrated and mounted after counterstaining, then the results were recorded and analyzed.

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