Protein was extracted from intestinal tissue and the protein concentration was measured. We loaded 10 ug of total protein onto SDS gel and transferred it to PVDF membranes and blocked it with nonfat milk. The primary antibody was applied overnight in a shaking table at 4°C. After extensive washing, the secondary antibody was applied for 1 h, finally, the protein bands were scanned and quantified by image development and ImageJ software.

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