The inhibitory effects of PSH on cell proliferation were measured by flow cytometry. Following the aforementioned treatments, U251 cells were collected and fixed with 70% ethanol at 4°C for 2 h. Following centrifugation (800 × g for 5 min at 4°C), the cells were resuspended in RNase A solution and 0.02 mg/ml PI (Sigma-Aldrich; Merck KGaA), and incubated for 30 min at 4°C. The fluorescence of 1×104 cells was recorded by a FACSCalibur C6 flow cytometer (BD Biosciences) and analyzed by ModFit software (Verity Software House).

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.