Iron oxide nanoparticles are prepared by simple co-precipitation method. Initially, 2 g of FeCl3.4H2O and 1 g of FeCl2.4H2O were dissolved in 50 ml of millipore water and kept in the ultrasonication bath at room temperature for 1 h. 0.1 M urea solution was slowly added drop wise and the sonication was continued for another 15 mins and resultant black precipitate was collected by centrifugation. The supernatant solution was decantated and the final product was dried in vacuum oven to get the black powdered iron oxide nanoparticles.

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