Before and after the cells were transfected with pGFPN1, pGFPN1/MIR181A2HG, pGFPN1/AKT2 or miRNA mimics, glucose in the cell culture medium was measured using Glucose Uptake Colorimetric assay kit (Sigma-Aldrich; MerckKGaA). Glucose uptake was calculated by the difference before and after the cells were treated. After the cells were treated, the glycogen level was detected using a glycogen assay kit (Nanjing Jiancheng Bioengineering Inc.), and the results were normalized to a standard curve and expressed as μg of glycogen, according to the manufacturer's instructions.

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