Matrigel gel was placed in a 24-well plate at 40 μl per well in a 37°C incubator for 2 h. After the cells were properly treated, HUVECs were digested and the cell density was adjusted to 3×105/ml. The cell suspension was added to the surface of Matrigel gel and placed in a 37°C incubator. The degree of tube formation was evaluated using a microscope after 24 h incubation at 37°C and 5% CO2. The full capillary-like structures in the field were counted and collected using a Canon EOS 600d camera.

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