HUVECs were treated with plasmids and 5.5 or 30 mM glucose. when the cells were 90-95% confluent, a sterile 200 μl pipette tip was used to draw straight lines in the middle of the well, and serum-free ECM medium was used to wash the cells twice, and this was then replaced with ECM medium containing 2% FBS, 1% PS and 1% EGF, as previously described (25). Images were collected (Canon EOS 600d) at this time as a 0 h control. The culture was continued for a further 24 h at 37°C. The image data were collected after 24 h.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.