RAW264.7, mouse peritoneal macrophages and bone marrow macrophages were seeded at a density of 1×105 cells/well in a 48-well plate overnight at 37°C and were then treated with various concentrations of WCCP-A-b (0, 25, 50, 100 and 200 μg/ml) or 1 μg/ml LPS at 37°C for 24 h. The concentrations of TNF-α and IL-6 in the supernatants were assessed using the aforementioned TNF-α and IL-6 ELISA kits according to the manufacturer's instructions.

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