RAW264.7 cells, mouse peritoneal macrophages and bone marrow macrophages were seeded at a density of 1×105 cells/well in a 48-well plate overnight at 37°C and were then treated with various concentrations (0, 25, 50, 100 and 200 μg/ml) of WCCP-A-b or 1 μg/ml LPS at 37°C for 24 h. After incubation, supernatants were collected and reacted with Griess reagent as previously described (36).

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.