For ER staining experiments, 0.5 μl of ER-tracker green DMSO stock solution (1 mM) was added into 2 ml of pre-warmed DMEM incubated at 37°C, 5% CO2 at room temperature for 30 min and washed with PBS solution 3 times. For Hoechst 33342/PI (CA1120, Beijing Solarbio Science & Technology Co., Ltd.) dual-staining, CHs were firstly stained with Hoechst 33342 (10 μM) for 30 min at 37°C and then stained with PI (10 μM) for 5 min. Cells were observed under fluorescence microscope (DMI4000B, Leica Microsystems, Inc.) after washing with PBS.

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