Cells were fixed with 4% paraformaldehyde and pre-incubated with 10% normal goat serum (KPL) at room temperature and then incubated with primary antibody anti-AKT2 (mouse monoclonal, 1:100, ab175354, Abcam) at 4°C overnight. Secondary antibody was FITC-labeled antibody to mouse IgG (cat. no. 5230-0307, KPL, 1:50, US) and incubated at room temperature for 2 h. Antifading mounting medium (with DAPI) (Beijing Solarbio Science & Technology Co., Ltd.) was used for nuclear staining at room temperature and stored at 4°C. Images were captured using a confocal microscope (Leica Microsystems GmbH) and digitized using LAS AF software (v. 2.6.0, Leica Microsystems GmbH).

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