The hPDLSCs were seeded into a 6-well plate with CM and transfected with siRNA targeting NLRP3 (si-NLRP3 forward, 5′-CCUCGGUACUCAGCACUAATT-3′ and reverse, 5′-UUAGUGCUGAGUACCGAG-3′; 20 μM) or negative control (si-NC forward, 5′-UUCUCCGAACGUGUCACGUTT-3′ and reverse, 5′-ACGUGACACGUUCGGAGAATT-3′; 20 μM) (Shanghai GenePharma Technology Co., Ltd.) with Micropoly-transfecter™ cell reagent (#MT115; Nantong Micropoly Biotech Co., Ltd.) when the cells were at a density of 70-80%. Following 24 h of treatment, the proteins were extracted to examine the interference efficiency.

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