RAW264.7 cells were purchased from the American Type Culture Collection. Cells were cultured in DMEM (Gibco; Thermo Fisher Scientific, Inc.) high glucose medium supplemented with 100 U/ml penicillin, 100 μg/ml streptomycin and 10% heat-inactivated FBS (Gibco; Thermo Fisher Scientific, Inc.). RAW264.7 cells were maintained at 37°C in a 5% CO2 incubator.

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