The TargetScan prediction algorithm ( to predict the binding between MIR181A2HG, miRNAs and AKT2. Luciferase reporter assay was performed as previously described (22). In brief, 293 cells were seeded into a 24-well plate and pmirGLO reporter plasmids (WT or mutant) or their empty plasmid were co-transfected with the miRNA mimic (GenePharma, Inc.) or miR-Ctl (GenePharma, Inc.) and pRL-TK. Following 24 h of transfection, luciferase activity was measured using a Dual-Glo Luciferase Assay System (Promega Corporation) with a Flash and Glow reader. The luciferase activity was expressed as the relative activity ratio of Firefly luciferase to Renilla luciferase. The sequences of MIR181A2HG are presented in Table SI.

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