The dome-Gal4 line was crossed with appropriate hhF4f-GFP;UAS-RNAi lines, mid-third instar larvae were collected and lymph glands were dissected lymph glands. Immunostaining was performed as described previously [14]. The following antibodies were used to identify PSC cells: mouse anti-Antp (primary antibody; 1:100; 4C3, Developmental Studies Hybridoma Bank); Alexa 555-conjugated mouse IgG antibody (secondary antibody; A28180, Thermo Fisher Scientific). Cell nuclei were stained with DAPI (Invitrogen). Immunostained samples were analysed with a Nikon A1R laser-scanning confocal microscope.

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