A total of 52 tissues from patients with HNSCC were collected from The Hospital of Stomatology, Sun Yat-Sen University (Guangzhou, China) between October 2017 and June 2018. No patient had received radiotherapy or chemotherapy before the biopsy, and all patients were diagnosed with HNSCC. The tissue samples were frozen at -80°C until required for use in further experiments. The histology and pathology of all samples was examined by two independent pathologists. Written informed consent was provided from all patients prior to participation and the study was approved by the Ethics Committee of Sun Yat-Sen University [approval no. ERC-(2017)-32].

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