Monosaccharide composition was determined using HPLC as described by Zhang et al (33). Briefly, polysaccharide samples (2 mg) were first hydrolyzed with 1 ml anhydrous methanol containing 2M HCl at 80°C for 16 h and then with 1 ml 2M trifluoroacetic acid at 120°C for 1 h. Following derivatization with 1-phenyl-3-methyl-5-pyrazo-lone, the derivatives were analyzed using a Shimadzu HPLC system.

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