CHs (5×103 cells/well) were cultured in 96-well plates and exposed to 0.4 mg/ml MTT diluted in complete DMEM for 4 h at 37°C. Cells were washed with PBS and allowed to air-dry overnight. Formazan crystals were resuspended in DMSO and absorbance was measured at 560 nm with background at 670 nm on a Cytation 5 plate reader (BioTek Instruments, Inc.).

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