Passage 3 cells were digested using trypsin, followed by washing using PBS. A total of 100 μl of prepared cell suspension was then incubated with antibodies (FITC mouse anti-human CD90, 5 μl, PE mouse anti-human CD44, 5 μl, PerCP-Cy™5.5 mouse anti-human CD105, 5 μl, APC mouse anti-human CD73, 5 μl, PE hMSC isotype control negative cocktail, 20 μl, PE hMSC negative cocktail, 20 μl, hMSC isotype control positive cocktail, 20 μl, hMSC positive cocktail, 20 μl, #562245, BD Biosciences) conjugated with a monoclonal fluorescent dye in the dark for 20 min at 4°C. Cells were detected using flow cytometry, as per the manufacturer's protocol (#660519, BD Biosciences). The MSC-associated positive cocktail, including CD105-PerCP-Cy, CD73-APC, CD44-PE, CD90-FITC and MSC-associated negative cocktail that included CD34 PE, CD45 PE, CD19 PE, HLA-DR PE and CD11b PE (#562245, BD Biosciences) were used. Respective negative and positive isotype control cocktails were used as systemic controls. Final database analysis was carried out using FlowJo software (Beckman coulter, Inc.).

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