The hPDLSCs were cultured in a 10-cm diameter culture plate (1,000 cells per plate) in α-MEM supplemented with 10% FBS [common medium (CM)]. After 7 days, the hPDLSCs were rinsed thrice using PBS, then fixed in 4% polyformaldehyde, after which they were stained with 0.1% crystal violet (Beijing Solarbio Science & Technology Co., Ltd.) at room temperature for 15 min. The cell clones were observed under a microscope (Olympus Corp.), and aggregates of >3 mm were considered as clones.

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