Fruiting bodies of C. cibarius were purchased from the Guilin Road Market (Changchun, China) and were identified using rDNA-Internal Transcribed Spacer sequencing analysis (27). Anion-exchange chromatography (DEAE-cellulose) was purchased from Amersham (Cytiva). Sepharose CL-6B was purchased from Cytiva, while LPS and Polymyxin B sulfate (PMB) were obtained from Sigma-Aldrich (Merck KGaA). TNF-α (cat. no. EK0527) and IL-6 ELISA kits (cat. no. EK0411) were obtained from Boster Biological Technology. Nembutal was acquired from Sinopharm Chemical Reagent Co., Ltd. SB203580 (cat. no. S1863), U0126 (cat. no. S1901) and SP600125 (cat. no. S1876) were acquired from Beyotime Institute of Biotechnology. All of the other reagents were of analytical grade or higher.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.