The human ESCC cell lines KYSE-410 and KYSE-30 were purchased from Cell Cook Company and the normal esophageal epithelial cell line (HEEC) was purchased from BeNa Culture Collection; Beijing Beina Chunglian Biotechnology Research Institute. Cells were cultured using RPMI-1640 medium (Gibco; Thermo Fisher Scientific, Inc.) supplemented with 10% FBS(Gibco; Thermo Scientific, Inc.) and cells were maintained at 37°C in an incubator containing 5% CO2.

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