Human CC cell lines (HeLa, SiHa, CaSki and C33A) were purchased from The Cell Bank of Type Culture Collection of The Chinese Academy of Sciences. SiHa, C33A and CaSki cells were cultured with RPMI-1640 medium (HyClone; Cytiva) containing 10% FBS (Biosera) and 1% penicillin-streptomycin (Beyotime Institute of Biotechnology). HeLa cells were cultured with 10% FBS and 1% penicillin-streptomycin in DMEM (HyClone; Cytiva). HeLa, SiHa, CaSki and C33A cells grown on a 6-well plate (30-50% confluence) were transfected with siRNA (100 nM per well) using Lipofectamine® 3000 (Invitrogen; Thermo Fisher Scientific, Inc.) according to the manufacturer's protocols. Transfected cells were incubated at 37°C and 5% CO2 for 2-4 days for subsequent experiments. The chemically synthesized GMPS specific double-stranded siRNA targeting sequences were as follows: Control (Ctrl) siRNA sense, 5′-UGGUUUACAUGUCGACUAATT-3′ and antisense, 5′-UUAGUCGACAUGUAAACCATT-3′; siRNA#1 sense, 5′-GCAUGUGUCACAACGUUAACA-3′ and antisense, 5′-UUAACGUUGUGACACAUGCGA-3′; siRNA#2 sense, 5′-CGAAUUAUGUAUGACUUAACA-3′ and antisense, 5′-UUAAGUCAUACAUAAUUCGAG-3′; and siRNA#3 sense, 5′-UGGAGAUAGUGUAGACAAAGU-3′ and antisense, 5′-UUUGUCUACACUAUCUCCAUG-3′. The siRNAs were synthesized by Shanghai Integrated Biotech Solutions Co., Ltd.

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