Skim milk agar was prepared as described previously [98]. Strains were inoculated into 2mL of TSB from blood agar plates and incubated to an OD600 of 0.4. 10μL of the bacterial culture were inoculated on a 12mm filter disk placed on the skim milk agar. Proteolysis zones on the agar were measured after 24h and 48h. For analysis proteolysis was determined as either positive (proteolysis zone around filter disk clearly visible) or negative, as measured diameters did not follow a symmetric distribution. Experiments were conducted in duplicates. Biological replicates were averaged for statistical analysis.

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