We analysed male mating success with respect to male genotype, female genotype and Rho B staining using logistic regression with a log link function to calculate the rate ratio and likelihood ratio estimates with 95% confidence intervals (CI) using JMP Pro version 15.1.0. The differences in time to locate a human host was analysed using one-way ANOVA with Tukey HSD post-hoc analysis. The wing beat frequency data was analysed using two-way ANOVA with Tukey HSD post-hoc analysis. Data were tested for normality using the Anderson-Darling test. All analyses were undertaken using GraphPad Prism version 7.00 unless otherwise stated. The numerical data used in all figures are included in S1 Data.

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