Pools of 10 female mosquitoes, 3–4 days old, from the S-Cairns, R-TL and R-BC strains were deprived of sugar solution for 24 h before testing for avidity. Starved females were individually transferred into a rearing cage, allowed to rest for 10 min post-transfer, and offered a blood meal from the forearm of a human adult volunteer for 5 min between 1700 and 1730 h. The number of fully or partially engorged females was recorded. Three batches of 10 mosquitoes from each strain were used for blood-feeding experiments and offered blood from the arm of the same human volunteer.

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