Two strains of Ae. aegypti were used to create an insecticide-resistant backcrossed strain with a double homozygous kdr genotype isolated in an otherwise fully susceptible background. R-TL is a pyrethroid-resistant strain that originated from Dili, Timor-Leste in 2009 [25], and S-Cairns is an insecticide susceptible reference strain of Ae. aegypti collected in Cairns, Australia in 2014. The derived backcross (R-BC) carries the homozygous mutations V1016G and S989P from the parental R-TL strain in a susceptible S-Cairns background. These kdr mutations are found in the same domain of the VGSC gene [10] and displayed complete linkage disequilibrium during the backcrossing process [21]. R-BC is resistant to DDT, permethrin, deltamethrin and lambda-cyhalothrin and susceptible to malathion and bendiocarb. The development of the R-BC strain and its genetic and phenotypic characterisation have already been described [21].

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