All the fish cell lines utilized were maintained as previously described for the PAC-2 cells [25,60]. The zebrafish embryonic cell line PAC-2 [61], cavefish embryonic cell line EPA [29], zebrafish adult fin cell line AB9 [36] and cavefish adult fin cell line CF1 [25] were cultured in L-15 (Leibovitz) medium (Gibco BRL) supplemented with 15% fetal Calf Serum (Sigma Aldrich F7524), 100 units/ml penicillin, 100 μg/ml streptomycin and 50 μg/ml gentamicin (Gibco BRL). Cells were maintained in an atmospheric CO2, non-humidified cell culture incubator at 26°C.

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