We tested the performance of VFOS in several conditions in which four cases were selected as representative examples: i) all barcode factors (GCC, HP, SR, HD, and CP) were considered; ii) only barcode factors GCC and HD were included; iii) only barcode factor GCC was examined; and iv) only barcode factor HD was considered in the test. The first condition was tested as a default setting of the VFOS program, and the remaining three conditions were examined as examples of user-defined settings. All results were obtained through 1000 repetitions of each condition.

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