Laboratory performances were assessed in agreement with the ISO 13528 standard, by considering the number of concordant, i.e. correct, results from qualitative testing. The result of a participating laboratory for a considered test (FAT, RTCIT, real-time RT-PCR, conventional RT-PCR) was considered “correct” when the laboratory detected the presence or absence of the lyssavirus in the positive and negative samples respectively (lyssavirus antigen for FAT, infectious live lyssavirus for RTCIT, lyssavirus RNA for conventional RT-PCR and real-time RT-PCR). A result was defined as “incorrect” when a discordant result occurred for a considered test (positive sample not found positive or negative sample not found negative). The evaluation of the diagnostic conclusion was considered “correct” if the diagnostic conclusion for the sample was concordant with the sample’s disease status. If an incorrect result occurred, the diagnostic conclusion was considered “incorrect”.

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