The probability of barcode exclusion (PEX) is determined as shown in Eq (2), which is composed of the total penalty score for a barcode (PTi = w1 × PGCCi + w2 × PHPi + w3 × PSRi + w4 × PHDi + w5 × PCPi), PWT, N, and α.

A single barcode factor or a certain combination of the factors can be used as a monitoring target(s) during a program run by providing a user’s own penalty scores and weights. As all barcodes can be excluded with their own probabilities, depending on penalty scores, the algorithm used in the VFOS program avoids instances in which a barcode set falls into a local minimum depending on an initial state.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.