Lotus japonicus seeds were scarified, subjected to water absorption for 30 min, and sown in quartz sand. The seeds were then germinated at 25°C and grown in a plant growth chamber with daily cycle of 16 hours of light at 25°C and 8 hours of dark at 23°C (BioTRON; Nippon Medical & Chemical Instruments Co., Ltd.). The plants were fertilized with 1000-fold diluted Hyponex® solution (N:P:K = 6:10:5) once a week. One-month-old L. japonicus were used in extracting plant hormones.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.